Immune
System
January
2016 By Kira A. Samoilova, PhD, DSci, Prof, International leader
in Photobiology and Medicine
Head
of Photobiology Unit Institute of Cytology of Russian Academy of
Sciences, St. Peterburg, Russia
18
YEARS OF INTENSIVE STUDY INTO BIOPTRON
The
experience of our laboratory investigation in the field of Photobiology
and Photomedicine exceeds 50 years, while the period of intensive
studies on BIOPTRON therapy is as long as 18 years. Our great interest
to this phototherapeutic modality is based on the unique properties
of BIOPTRON light that simulates dominant components of the terrestrial
solar radiation polychromatic visible and infrared radiation
with power density, characteristic of a summer days in Europe.
These 2 parts of solar spectrum occupy about 97% of the solar radiation
on the Earths surface. Hence, we deal with a very important
environmental factor, which allows us to consider reactions to the
light of human and animal organisms as an adoptive response to the
light exposures that was developed during a long period of evolution.
For the last several years, we studied the effect of BIOPTRON light
on the blood properties that are important for regenerative and
metabolic processes. Because the blood movement rate in circulation
is determined by red blood cells, we investigated their rheological
properties. It was shown that in 0.5-24 hrs after a single irradiation
of volunteers with deformability, red blood cells increased, while
their viscosity fell. Simultaneously the transport function, (in
particular, oxygen transport) enhanced, which resulted to increase
of partial oxygen pressure in blood.
In
parallel, disaggregation of platelets and increase of anti-coagulation
activity of plasma components were observed, which seems to determine
the development of anti-thrombotic effect of BIOPTRON-light: irradiation
of the rat femoral arteries completely blocked (prevented) development
in this vessels of the experimentally induced irrevesal thrombosis.
An
important role in trophic function of blood belongs to its circulation
rate in micro-vessels. According to our observation, as soon as
2 minutes after irradiation of a small body area the rate of microcirculation
in volunteers and patients with Type II diabetes mellitus increased
both locally and in remote tissues (i.e. at the systemic level).
The optimum increase of microcirculation rate was observed at 30
minutes, (up to 47%).
The
evidence was obtained in our study that in both cases the increase
of microcirculation rate resulted from activation of synthesis of
nitric oxide (NO) the most important vasodilatator that is
secreted by vascular endothelial cells and platelets.
Apart
from the improvement of microcirculation and enhancement of the
transport function of blood, the correction of some indices of metabolic
processes was recorded: after exposure to BIOPTRON light in volunteers
blood the level of glucose and atherogenic lipids (triglycerides,
cholesterol, ß-lipoproteins) fell, while the content of anti-atherogenic
lipids, (a-lipoproteins) increased.
Wound
healing effects of BIOPTRON light therapy is undoubtedly associated
with improvement of blood microcirculation, enhancement of the trophic
function of blood, but also with increase of concentration in blood
serum of growth factors and some cytokines.
We
had also demonstrated, that addition to culture media of 2.5% of
serum, isolated from blood of volunteers or patients with Breast
Cancer I-II stages after 7-10 daily post-surgery exposures to BIOPTRON
light, significantly stimulated proliferation of keratinocytes,
endotheliocytes and fibroblasts basic participants of wound healing
process, but inhibited proliferation of several lines of human tumor
cells.
In
experiments with laboratory animals, it was shown that exposure
to BIOPTRON light decelerates the growth of malignant tumors (murine
hepatoma) both after light treatment of mice with tumors, and after
direct exposure to light of tumor cells themselves with their subsequent
transplantation to syngeneic mice.
The mechanism of anti-tumor effect of BIOPTRON light was not associated
with cytotoxic or cytostatic action of light on cells, but was a
consequence of structural changes of tumor cell surface which enhanced
their recognition by natural killer cells main effectors
of the innate anti-tumor immunity.
As
a consequence, cytolitic activity of natural killer cells increased
which resulted in the death of the light-irradiated tumor cells.
Mechanism of anti-tumor effect of BIOPTRON light in case of photoirradiation
of tumor-bearing mice needs to be studied in the future. However,
in our opinion the oncological safety of BIOPTRON light therapy
has been already proved.
All
the above data was published in main international journals on Photomedicine
and Photobiology (Photomedicine and Laser Surgery, Photochemical
and Photobiological Sciences, Photochemistry and Photobiology, Laser
Therapy, Photodiagnosis and Photodynamic therapy, Lasers in Medical
Sciences etc).
SHORT
INFORMATION:
For 18 years of intensive studies of BIOPTRON light effects in human
we could elucidate mechanisms of main systemic effects anti-inflammatory,
immunomodulatory, wound healing, anti-tumor and normalization of
metabolic processes. These effects are developing due to transcutaneous
photomodification of blood in the upper skin vasculature. It is
noteworthy, that irradiation of small area of the body surface leads
to changes in the entire circulating blood volume. It is undoubtedly
associated with unique physical peculiarities of BIOPTRON light:
its polychromatic visible and infrared components simulates spectral
and power density parameters of two dominant kinds of the terrestrial
Solar radiation - the main environmental factor. During evolution
they could promote the development in living organisms the adoptive
beneficial mechanisms of light utilization.
Reference
List, (Prof. Samoilova et al.)
1.
Samoilova K.A., Obolenskaya K.D, Vologdina A.V., Snopov S.A., Shevchenko
E.V. Single skin exposure to visible polarized light induces rapid
modification of entire circulating blood. 1. Improvement of rheologic
and immune parameters. Proc. SPIE. 1998. Vol. 3569.
P. 90-103.
2. Samoilova K.A., Zubanova O.I., Snopov S.A., Mukhuradze N.A.,
Mikhelson V.M. Single skin exposure to visible polarized light induces
rapid modification of entire circulating blood. 2. Appearance of
soluble factors restoring proliferation and chromosome structure
in X-damaged lymphocytes. Proc. SPIE, 1998, 3569: 26-33.
3. Zhevago N.A., Samoilova K.A., Glazanova T.V., Pavlova I.E., Bubnova
L.N., Rosanova O.E., Obolenskaya K.D. Exposures of human body surface
to polychromatic (visible + infrared) polarized light modulate a
membrane phenotype of the peripheral blood mononuclear cells. Laser
Technology. 2002. Vol. 12 (1). P. 7-24.
4. Obolenskaya K.D., Samoilova K.A. Comparative study of effects
of polarized and non-polarized light on human blood in vivo and
in vitro. I. Phagocytosis of monocytes and granulocytes. Laser Technology.
2002 Vol. 12(2-3). P.7-13.
5. Zhevago N.A., Samoilova K.A., Obolenskaya K.D. The regulatory
effect of polychromatic (visible and infrared) light on human humoral
immunity. Photochemical and Photobiological Sciences 2004.
Vol. 3, ?.1. P.102-108.
6. Samoilova K.A., Bogacheva O.N., Obolenskaya K.D., Blinova M.I.,
Kalmykova N. V., Kuzminikh E.V. 2004. Enhancement of the blood growth
promoting activity after exposure of volunteers to visible and infrared
polarized light. I. Stimulation of human keratinocyte proliferation
in vitro. Photochemical and Photobiological Sciences 2004.
Vol. 3, ?.1. P.96-101.
7. Bogacheva ON, Samoilova KA, Zhevago NA, Obolenskaia KD, Blinova
MI, Kalmykova NV, Kuz'minykh EV.Enhancement of fibroblast growth
promoting activity of human blood after its irradiation in vivo
(transcutaneously) and in vitro with visible and infrared polarized
light. Tsitologiia. 2004. Vol.46(2).
159-171.
8. Zhevago N.A., Samoilova K.A. Pro- and anti-inflammatory cytokine
content in the human peripheral blood after its transcutaneous and
direct (in vitro) irradiation with polychromatic visible and infrared
light. Photomedicine and Laser Surgery. 2006. Vol.
24(2). P.129-139.
9. Zhevago N.A., Samoilova K.A., Calderhead R.G. Polychromatic light
similar to the terrestrial solar spectrum without its UV component
stimulates DNA synthesis in human peripheral blood lymphocytes in
vivo and in vitro. Photochemistry Photobiology. 2006.
Vol. 82(5). P.1301-1308.
10. Knyazev NA., Samoilova KA, Filatova NA, Galaktionova AA. Effect
of polychromatic light on proliferation of tumor cells under condition
in vitro and in vivo after implantation to experimental animals.
Proc. SPIE. 2009. Vol.1142. P.79-86.
11. Zhevago NA, Samoilova KA, Davydova NI, Bychkova NV, Glazanova
TV, Chubukina ZhV, Buiniakova AI, Zimin AA.The efficacy of polychromatic
visible and infrared radiation used for the postoperative immunological
rehabilitation of patients with breast cancer. Vopr Kurortol Fizioter
Lech Fiz Kult. 2012. Vol.4. P.23-32.
12. Filatova N.A., Knyazev N.A., Kosheverova V.V, Shatrova A.N.,
Samoilova K.A. The effect of radiation with polichromatic visible
and infrared light on the tumorigenicity of murine hepatoma 22A
cells and their sensitivity to lysis by natural killers. Cell and
Tissue Biology. 2013. Vol.7(6). P. 573-577.
13. Knyazev NA, Filatova NA, Samoilova KA. Proliferation and tumorigenity
of murine hepatoma cells irradiated with polichromatic visible and
infrared light. Cell and Tissue Biology. 2013. Vol.7(1).
P.79-85.
Samoilova KA, Zimin AA, Buinyakova AI, Makela AM, 14. Zhevago NA.
Regulatory systemic effect of postsurgical polychromatic light (480-3400?nm)
irradiation of breast cancer patients on the proliferation of tumor
and normal cells in vitro. Photomedicine and Laser Surgery.
2015. Vol. 33(11). P.555-563.
15. Knyazev NA, Samoilova KA, Abrahamse H, Filatova NA. Downregulation
of tumorogenicity and changes in the actin cytoskeleton of murine
hepatoma after irradiation with polychromatic visible and IR light.
Photomedicine and Laser Surgery. 2015. Vol.
33(4). P.185-192.
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Steven Warren - Light Therapy UK. All rights reserved 2005 - 2018.
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